QTL Detection for Leaf Morphology of Rice Using Chromosome Segment Substitution Lines

doi:10.3969/j.issn.10017216.2013.01.004

Abstract

Abstract: The morphological characteristic is one of profitable targets in rice promotion of plant type and yield. A population of chromosome segment substitution lines (CSSLs) derived from backcross between indica recipient 9311 and japonica donar Nipponbare was employed to map quantitative trait loci for the top three leaf size (i.e., leaf length, width and area) and main panicle yield (i.e., spikelet number of main panicle, 1000grain weight of main panicle, seed setting rate of main panicle, main panicle weight), and analyzed their correlation between leaf and yield traits. Correlation analysis revealed that there were significant correlation among traits of leaf size in CSSLs population. Traits of leaf morphology were significantly positively correlated with main panicle weight. Most of traits of leaf morphology had no correlation with seed setting rate, 1000grain weight. A total of 20 QTLs for the top three leaf size were located at 10 regions on 7 chromosomes except for chromosomes 2, 7, 8, 10 and 12 in two years, with explained phenotypic variations ranging from 3.82% to 14.61% and that of six ones were above 10%. Various QTLs were distributed in cluster, and three QTLs were detected in both two years. Furthermore, eight QTLs were novel QTL, comparing with the other mapping results. A total of 16 QTLs for the traits of main panicle yield were detected at 13 regions on 7 chromosomes of 1, 2, 3, 5, 7, 8 and 10 in two years, and 5 regions were in common with QTLs of leaf morphology and yield.

Key words: rice, chromosome segment substitution lines, quantitative trait loci, leaf morphology, yield, main panicle

摘要： 水稻叶片的形态改良是水稻株型育种和产量育种的重要目标之一。以9311/日本晴染色体片段置换系（CSSLs）群体为材料，定位了上3叶叶片长、宽、叶面积共9个性状QTL，分析了叶片性状与产量性状之间的相关性，同时定位了主穗重及产量构成因素（颖花数、千粒重、结实率）相关QTL。结果表明，CSSLs群体的叶片性状之间存在显著或极显著相关性；叶片性状与主穗重呈显著或极显著正相关，与主穗颖花数呈极显著正相关；叶片形态多数性状与结实率、千粒重没有显著相关性。两年共定位到20个叶片性状QTL，分布于第1、3、4、5、6、9、11共7条染色体的10个区间，贡献率为3.82%~14.61%，其中贡献率大于10%有6个，多个QTL成簇分布在相同区间，3个QTL在两年间重复检测到，8个QTL为前人未报道的新位点。两年共检测到16个与控制主穗产量相关的QTL，分布于第1、2、3、5、7、8、10共7条染色体13个区间，其中有7个主穗产量相关QTL所在5个区间与叶片形态14个QTL所在区间一致。

关键词: 水稻, 染色体片段置换系, 数量性状基因座, 叶片形态, 产量, 主穗

CLC Number:

ZHOU Lihui, ZHAO Chunfang, ZHAO Ling, ZHANG Yadong, ZHU Zhen, CHEN Tao, ZHAO Qingyong, YAO Shu, YU Xin, WANG Cailin*. QTL Detection for Leaf Morphology of Rice Using Chromosome Segment Substitution Lines[J]. Chinese Journal of Rice Science, 2013, 27(1): 26-34.

周丽慧,赵春芳,赵凌,张亚东,朱镇,陈涛,赵庆勇,姚姝,于新,王才林*. 利用染色体片段置换系群体检测水稻叶片形态QTL[J]. 中国水稻科学, 2013, 27(1): 26-34.

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